This product is a bacteriostatic antibiotic, but it also has bactericidal effect on sensitive bacteria at very high concentration. The antibacterial mechanism of action is to inhibit bacterial protein synthesis by binding to the 50s subunit of the bacterial ribosome.

Tiamulin hydrogen fumarate has good antibacterial activity against a variety of Gram-positive cocci, including most staphylococci and streptococci (except group D streptococci) and a variety of mycoplasmas and some spirochetes. However, the antibacterial activity against some negative bacteria is very weak, except for Haemophilus species and some E. coli and Klebsiella strains.

Tiamulin hydrogen fumarate is easy to be absorbed after oral administration in pigs. About 85% of a single dose is absorbed, and peak concentrations occur in 2 to 4 hours. It is widely distributed in the body, with the highest concentration in the lungs. Tiamulin hydrogen fumarate is metabolized in the body into 20 metabolites, some with antibacterial tranquility. About 30% of the metabolites are exted in the urine and the remainder is exted in the feces.