Wannan kit ɗin yana amfani da tsarin buffer na musamman wanda zai iya sakin RNA da sauri daga samfuran ƙwayoyin halitta don halayen RT-qPCR, ta haka ne ke kawar da tsarin tsarkakewar RNA mai cin lokaci da wahala.Ana iya samun samfurin RNA a cikin mintuna 7 kawai.5 × Direct RT Mix da 2 × Direct qPCR Mix-SYBR reagents wanda kit ɗin ke bayarwa na iya samun sakamako mai ƙima na PCR cikin sauri da inganci.

5 × Direct RT Mix da 2 × Direct qPCR Mix-SYBR suna da ƙarfin juriya mai hanawa, kuma ana iya amfani da lysate na samfuran azaman samfuri don RT-qPCR kai tsaye.Wannan kit ɗin ya ƙunshi keɓaɓɓen RNA high-affinity Foregene reverse transcriptase, da Hot D-Taq DNA polymerase, dNTPs, MgCl.₂, amsawa buffer, PCR ingantawa da stabilizer.

200 × 20μl Rxns, 1000 × 20μl Rxns

■ Sauƙi kuma mai tasiri: tare da fasahar Cell Direct RT, ana iya samun samfuran RNA a cikin mintuna 7 kacal.

∎ Bukatar samfurin karami ne, kamar yadda za a iya gwada ƙananan sel guda 10.

∎ Babban abin da ake fitarwa: zai iya gano RNA da sauri a cikin sel waɗanda aka tsara a cikin faranti 384, 96, 24, 12, 6-rijiya.

∎ Gogewar DNA na iya saurin cire kwayoyin halittar da aka fitar, da rage tasirin sakamakon gwaji na gaba.

∎ Ingantaccen tsarin RT da qPCR yana sa jujjuyawar matakai biyu na RT-PCR mafi inganci kuma PCR ta fi ƙayyadaddun ƙayyadaddun bayanai, kuma mafi juriya ga masu hana amsawar RT-qPCR.

Iyakar aikace-aikace: sel masu al'ada.

- RNA da aka saki ta samfurin lysis: kawai ana amfani da samfurin RT-qPCR na wannan kit.

- Za a iya amfani da kit ɗin don dalilai masu zuwa: nazarin maganganun kwayoyin halitta, tabbatar da tasirin sirna mai tsaka-tsaki na yin shiru, gwajin magunguna, da sauransu.