Jimlar Dabbobin Keɓewar RNA
Shirye-shirye 50, Shirye-shiryen 200
Wannan kit yana amfani da shijuya shafi da dabaraKamfaninmu ya haɓaka, wanda zai iya fitar da tsafta mai ƙarfi da inganci duka RNA daga kyallen dabbobi daban-daban tare da ingantaccen aiki.Yana ba da ingantaccen ginshiƙi na tsaftacewa na DNA, wanda zai iya rarrabewa da kuma tallata DNA na genomic daga mai ƙarfi da lysate nama, mai sauƙi. da tanadin lokaci;Rukunin RNA-kawai na iya ɗaure RNA da kyau da kyau kuma ana iya sarrafa su lokaci guda tare da keɓancewar dabara Yawancin samfura.
Dukkanin tsarin ba shi da RNase-Free, don kada RNA da aka fitar ba ta ƙasƙanta ba;Buffer RW1, Tsarin wanke buffer RW2, ta yadda RNA da aka samu ba ta da furotin, DNA, ion, da gurɓataccen mahalli.
Abubuwan Kit
| Jimlar Dabbobin Keɓewar RNA | ||
| Abubuwan Kit | RE-03011 | RE-03014 |
| 50 T | 200 T | |
| RL1* | ml 25 | 100 ml |
| Farashin RL2 | ml 15 | ml 60 |
| RW1* | ml 25 | 100 ml |
| Farashin RW2 | 24ml ku | ml 96 |
| RNase-Free ddH2O | ml 10 | ml 40 |
| Rukunin RNA-kawai | 50 | 200 |
| Rukunin Tsabtace DNA | 50 | 200 |
| Jagoran Jagora | guda 1 | guda 1 |
Bayanin samfur
| Tsarin | Juya shafi | Bangaren tsarkakewa | Rukunin Foregene, reagent |
| Flux | 1-24 samfurori | Lokaci kowane shiri | ~ 30 min (samfurori 24) |
| Centrifuge | Tebur centrifuge | Pyrolysis rabuwa | Rabuwar Centrifugal |
| Misali | Naman dabba;tantanin halitta | Adadin samfuran | Nama: 10-20 MG;Cell: (1-5)×106 |
| Ƙarar hasashe | 50-200 ml | Matsakaicin ƙarar lodi | 850 ml |
Fasaloli & fa'idodi
■ Babu buƙatar damuwa game da lalacewar RNA;Duk tsarin ba shi da RNase-Free
∎ Cire DNA da kyau ta amfani da Rukunin Tsabtace DNA
■ Cire DNA ba tare da ƙara DNA ba
■ Ana kammala ayyuka masu sauƙi-duk a yanayin zafin ɗaki
■ Ana iya kammala aikin gaggawa cikin mintuna 30
∎ Amintacciya-babu reagent na halitta da ake buƙata
■ Babban tsarki -OD260/280≈1.8-2.1
Kit aikace-aikace
Ya dace da hakar da tsarkakewar jimillar RNA daga sabo ko daskararru iri-iri na kyallen dabbobi ko sel masu al'ada.
Siffofin samfur
∎ Aikace-aikace na ƙasa: haɗin cDNA na farko, RT-PCR, cloning na kwayoyin halitta, Northern Blot, da sauransu.
Samfurori: kyallen jikin dabba, ƙwayoyin al'ada
■ Adadin: Nama 10-20mg, Kwayoyin (2-5) × 106
■ Ƙarfin ɗaurin DNA na ginshiƙin tsarkakewa: 80 μg
■ Ƙarfin haske: 50-200 μl
Gudun aiki
Jimlar Kayan Keɓewar Dabbobin RNA da aka yi maganin 20mg
Sabbin samfuran linzamin kwamfuta, ɗauki 5% tsarkakakken jimlar RNA 1% agar
Glycogel electrophoresis
1: Baki 2: Koda
3: Hanta 4: Zuciya
Adana da rayuwar shiryayye
Ana iya adana kit ɗin na tsawon watanni 24 a zazzabi na ɗaki (15-25 ℃) ko 2-8 ℃ na tsawon lokaci.Ana iya adana buffer RL1 a 4 ℃ na wata 1 bayan ƙara β-mercaptoethanol (na zaɓi).
Labarai da aka kawo
1.Idan: 18.808:Zheng, Q., Qin, F., Luo, R., da al.mRNA-Loaded Lipid-Kamar Nanoparticles don Gyara Tushen Hanta Ta Hanyar Haɓaka Ƙirƙirar Ƙirƙirar Ƙirar Tsakiya.Adv.AyyukaMatar.2021, 31, 2011068.doi:10.1002/adfm.202011068.
2.Idan: 18.187:He X, Hong W, Yang J, et al.Kwatsam apoptosis na sel a cikin shirye-shiryen ƙwayar ƙwayar cuta yana yin tasirin immunomodulatory ta hanyar sakin phosphatidylserine.Canjin Siginar Target Ther.2021 Yuli 14; 6 (1): 270.doi: 10.1038/s41392-021-00688-z.
3.Idan: 17.97 
ai Z, Liu H, Liao J, et al.N7-Methylguanosine tRNA gyare-gyare yana haɓaka fassarar mRNA oncogenic kuma yana haɓaka ci gaban cholangiocarcinoma na ciki.Mol Cell.2021 Jul 29: S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.
4.Idan: 9.225: Cao X, Shu Y, Chen Y, et al.Mettl14-Matsakaici m6A Canjin Yana Sauƙaƙe Farfaɗowar Hanta ta hanyar Kula da Endoplasmic Reticulum Homeostasis.Cell Mol Gastroenterol Hepatol.2021;12 (2): 633-651.doi: 10.1016/j.jcmgh.2021.04.001.
RNA ware kayan aikin don sauran samfurin kafofinakwai:
Kwayoyin halitta, shuka, kwayar cuta, jini, da dai sauransu.
Kwararren injiniyan fasaha da aka sadaukar don jagorantar ku
Dangane da ainihin buƙatun ku, zaɓi mafi madaidaicin ƙira da hanyoyin tsarawa gabaɗaya