Awọn igbaradi 50, Awọn igbaradi 200

Ohun elo yii nloomo ọwọn ati agbekalẹti o ni idagbasoke nipasẹ ile-iṣẹ wa, eyiti o le jade ni mimọ-giga ati didara lapapọ RNA lati ọpọlọpọ awọn ẹran ara ẹranko pẹlu ṣiṣe giga.O pese iwe-itọju DNA ti o munadoko, eyiti o le ni rọọrun yapa ati adsorb DNA genomic lati supernatant ati lysate tissu, rọrun. ati fifipamọ akoko;Ọwọn RNA-nikan le di RNA daradara ati pe o le ṣe ilọsiwaju nigbakanna pẹlu agbekalẹ alailẹgbẹ Ọpọlọpọ awọn ayẹwo.

Gbogbo eto jẹ RNase-ọfẹ, ki RNA ti a fa jade ko ni ibajẹ;Buffer RW1, Buffer RW2 eto fifọ saarin, ki RNA ti o gba jẹ ọfẹ ti amuaradagba, DNA, ion, ati idoti agbo-ara Organic.

Awọn paati ohun elo

ọja alaye

Awọn ẹya & awọn anfani

■ Ko si ye lati ṣe aniyan nipa ibajẹ RNA;gbogbo eto jẹ RNase-ọfẹ
■ Yọ DNA kuro daradara-lilo DNA-Cleaning Column
■ Yọ DNA kuro lai fi DNAse kun
■ Rọrun-gbogbo awọn iṣẹ ṣiṣe ti pari ni iwọn otutu yara
■ Ṣiṣe-yara le pari ni ọgbọn išẹju 30
■ Ailewu-ko si reagenti Organic ti o nilo
■ Giga ti nw -OD260 / 280≈1.8-2.1

Ohun elo Kit

O dara fun isediwon ati ìwẹnumọ ti lapapọ RNA lati ọpọlọpọ awọn ẹran ara tuntun tabi tutunini tabi awọn sẹẹli gbin.

Ọja sile

■ Awọn ohun elo ti o wa ni isalẹ: iṣakojọpọ cDNA akọkọ-okun, RT-PCR, cloning molikula, Northern Blot, ati bẹbẹ lọ.
■ Awọn ayẹwo: awọn ẹran ara ẹranko, awọn sẹẹli ti a gbin
■ Iwọn lilo: Tissues 10-20mg, Awọn sẹẹli (2-5) × 10⁶
■ O pọju agbara abuda DNA ti ọwọn ìwẹnumọ: 80 μg
■ Iwọn didun: 50-200 μl

Sisan iṣẹ

Eranko Total RNA ipinya Apo itọju 20mg
Awọn ayẹwo Asin titun, mu 5% ti a sọ di mimọ lapapọ RNA 1% agar

Glycogel electrophoresis
1: Ọkàn 2: Àrùn
3: Ẹdọ 4: Okan

Ibi ipamọ ati igbesi aye selifu

Ohun elo naa le wa ni ipamọ fun oṣu 24 ni iwọn otutu yara (15–25 ℃) tabi 2–8 ℃ fun igba pipẹ.Buffer RL1 le wa ni ipamọ ni 4 ℃ fun oṣu kan lẹhin fifi β-mercaptoethanol kun (aṣayan).

 

Toka ìwé

1.TI: 18.808:Zheng, Q., Qin, F., Luo, R., ati al.mRNA-Ti kojọpọ Lipid-Bi awọn ẹwẹ ara fun Ṣiṣatunṣe Ipilẹ Ẹdọ Nipasẹ Imudara ti Apẹrẹ Apapo Aarin.Adv.Iṣẹ.Mater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.Ti: 18.187:He X, Hong W, Yang J, et al.Apoptosis lẹẹkọkan ti awọn sẹẹli ni igbaradi sẹẹli yio ṣe itọju awọn ipa immunomodulatory nipasẹ itusilẹ ti phosphatidylserine.Ifojusi Iyipada ifihan agbara Ther.Ọdun 2021 Oṣu Keje 14;6 (1):270.doi: 10.1038 / s41392-021-00688-z.

3.BI:17.97 ai Z, Liu H, Liao J, et al.N7-Methylguanosine tRNA iyipada ṣe alekun itumọ mRNA oncogenic ati ṣe igbega ilọsiwaju cholangiocarcinoma intrahepatic.Mol Cell.2021 Jul 29: S1097-2765 (21)00555-4.doi: 10.1016 / j.molcel.2021.07.003.

4.BI:9.225: Cao X, Shu Y, Chen Y, ati al.Mettl14-Mediated m6A Iyipada Ṣe Atunse Ẹdọ Rọ nipasẹ Mimu Endoplasmic Reticulum Homeostasis.Cell Mol Gastroenterol Hepatol.2021;12 (2): 633-651.doi: 10.1016 / j.jcmgh.2021.04.001.

 

Awọn ohun elo ipinya RNA fun awọn orisun apẹẹrẹ miiranwa:

Awọn sẹẹli, ọgbin, gbogun ti, ẹjẹ, ati bẹbẹ lọ.

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Ti tẹlẹ: Cell Direct RT QPCR Kit-SYBR GREEN I

Itele: Olupese ṣe agbejade fiimu ifojusọna egboogi-oorun

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