Animal Total RNA Isolation Kit
50 Preps, 200 Preps
This kit uses the spin column and formula developed by our company, which can extract high-purity and high-quality total RNA from various animal tissues with high efficiency.It provides an efficient DNA-Cleaning Column, which can easily separate and adsorb genomic DNA from the supernatant and tissue lysate, simple and time-saving; RNA-only Column can efficiently bind RNA and can be processed simultaneously with a unique formula Lots of samples.
The whole system is RNase-Free, so that the extracted RNA is not degraded; Buffer RW1, Buffer RW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ion, and organic compound pollution.
Kit components
| Animal Total RNA Isolation Kit | ||
| Kit components | RE-03011 | RE-03014 |
| 50 T | 200 T | |
| Buffer RL1* | 25ml | 100ml |
| Buffer RL2 | 15ml | 60ml |
| Buffer RW1* | 25ml | 100ml |
| Buffer RW2 | 24ml | 96ml |
| RNase-Free ddH2O | 10ml | 40ml |
| RNA-only Column | 50 | 200 |
| DNA-Cleaning Column | 50 | 200 |
| Instruction Manual | 1 piece | 1 piece |
Product information
| Format | Spin column | Purification component | Foregene column, reagent |
| Flux | 1-24 samples | Time per prep | ~30 min (24 samples) |
| Centrifuge | Desk centrifuge | Pyrolysis separation | Centrifugal separation |
| Sample | Animal tissue; cell | Samples amount | Tissue:10-20 mg; Cell:(1-5)×106 |
| Elution volume | 50-200 μL | Maximum loading volume | 850 μL |
Features&advantages
■ No need to worry about RNA degradation; the whole system is RNase-Free
■ Effectively remove DNA-using DNA-Cleaning Column
■ Remove DNA without adding DNase
■ Simple-all operations are completed at room temperature
■ Fast -operation can be completed in 30 minutes
■ Safe-no organic reagent required
■ High purity -OD260/280≈1.8-2.1
Kit application
It is suitable for the extraction and purification of total RNA from a variety of fresh or frozen animal tissues or cultured cells.
Product parameters
■ Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
■ Samples: animal tissues, cultured cells
■ Dosage: Tissues 10-20mg, Cells(2-5)×106
■ Maximum DNA binding capacity of purification column: 80 μg
■ Elution volume: 50-200 μl
Work flow
Animal Total RNA Isolation Kit treated 20mg
Fresh mouse samples, take 5% purified total RNA 1% agar
Glycogel electrophoresis
1: Spleen 2: Kidney
3: Liver 4: Heart
Storage and shelf life
The kit can be stored for 24 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time. Buffer RL1 can be stored at 4 ℃ for 1 month after adding β- mercaptoethanol (optional).
Cited articles
1. IF:18.808: Zheng, Q., Qin, F., Luo, R., et al. mRNA-Loaded Lipid-Like Nanoparticles for Liver Base Editing Via the Optimization of Central Composite Design. Adv. Funct. Mater. 2021, 31, 2011068.doi:10.1002/adfm.202011068.
2.IF:18.187:He X, Hong W, Yang J, et al. Spontaneous apoptosis of cells in therapeutic stem cell preparation exert immunomodulatory effects through release of phosphatidylserine. Signal Transduct Target Ther. 2021 Jul 14;6(1):270. doi: 10.1038/s41392-021-00688-z.
3.IF:17.97 
ai Z, Liu H, Liao J, et al. N7-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression. Mol Cell. 2021 Jul 29:S1097-2765(21)00555-4. doi: 10.1016/j.molcel.2021.07.003.
4.IF:9.225:Cao X, Shu Y, Chen Y, et al. Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cell Mol Gastroenterol Hepatol. 2021;12(2):633-651. doi: 10.1016/j.jcmgh.2021.04.001.
RNA isoaltion kits for other sample sources are available:
Cell, plant, viral, blood, etc.
Professional technical engineer dedicated to guide you
According to your actual needs, choose the most reasonable overall design and planning procedures