50 Mākaukau, 200 Mākaukau

Hoʻohana kēia pahu i kakolamu milo a me ka formulai hoʻomohala ʻia e kā mākou hui, hiki iā ia ke unuhi i ka RNA holoʻokoʻa kiʻekiʻe-maʻemaʻe a kiʻekiʻe loa mai nā ʻano holoholona like ʻole me ka hana kiʻekiʻe. a me ka mālama manawa;Hiki i ke kolamu RNA-wale ke hoʻopaʻa maikaʻi i ka RNA a hiki ke hana ʻia i ka manawa like me kahi ʻano kūʻokoʻa Nui nā laʻana.

ʻO ka ʻōnaehana holoʻokoʻa he RNase-Free, i ʻole e hoʻohaʻahaʻa ʻia ka RNA i unuhi ʻia;Buffer RW1, Buffer RW2 ʻōnaehana holoi holoi, no laila ʻo ka RNA i loaʻa ʻole i ka protein, DNA, ion, a me ka pollution hui.

Nā ʻāpana pahu

ʻIke huahana

Nā hiʻohiʻona & pono

■ ʻAʻole pono e hopohopo no ka hoʻohaʻahaʻa ʻana o RNA;ʻo RNase-Free ka ʻōnaehana holoʻokoʻa
■ Hoʻopau maikaʻi i ka DNA-hoʻohana i ka DNA-Cleaning Column
■ Wehe i ka DNA me ka hookomo ole i ka DNase
■ Maalahi-ua pau na hana a pau ma ka wela lumi
■ Hiki ke hoʻopau i ka hana wikiwiki i 30 mau minuke
■ Palekana-ʻaʻole pono ka mea hoʻoheheʻe organik
■ Maʻemaʻe kiʻekiʻe -OD260/280≈1.8-2.1

Ke noi pahu

He kūpono ia no ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana i ka RNA holoʻokoʻa mai nā ʻano ʻiʻo holoholona hou a maloʻo paha a i ʻole nā ​​​​cell moʻomeheu.

Nā palena huahana

■ Nā noi i lalo: ka hoʻohui ʻana o ka cDNA mua, RT-PCR, molecular cloning, Northern Blot, etc.
■ Nā laʻana: nā ʻiʻo holoholona, ​​nā pūnaewele moʻomeheu
■ Ka Papa Hana: Tissues 10-20mg, Cells(2-5)×10⁶
■ Ka hiki ke hoʻopaʻa DNA kiʻekiʻe o ke kolamu hoʻomaʻemaʻe: 80 μg
■ Ka nui elution: 50-200 μl

Ka holo hana

ʻO ka Animal Total RNA Isolation Kit i mālama ʻia he 20mg
Nā laʻana ʻiole hou, e lawe i ka 5% i hoʻomaʻemaʻe ʻia ka RNA 1% agar

ʻO ka electrophoresis glycogel
1: Spleen 2: Kidney
3: Ake 4: Puuwai

Ka mālama ʻana a me ka mālama ʻana

Hiki ke mālama ʻia ka pahu no 24 mau mahina ma ka lumi wela (15–25 ℃) a i ʻole 2–8 ℃ no ka manawa lōʻihi.Hiki ke mālama ʻia ka Buffer RL1 ma 4 ℃ no 1 mahina ma hope o ka hoʻohui ʻana i ka β-mercapptoethanol (ke koho).

 

Nā ʻatikala i haʻi ʻia

1.IF:18.808:Zheng, Q., Qin, F., Luo, R., et al.mRNA-Loaded Lipid-like Nanoparticles no ka hoʻoponopono ʻana i ke ʻano o ke akepaʻa ma o ka hoʻolālā ʻana o ka Central Composite Design.ʻO Adv.Hana.Mater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.IF:18.187:He X, Hong W, Yang J, et al.ʻO ka apoptosis maʻamau o nā pūnaewele i loko o ka hoʻomākaukau ʻana i ka cell stem therapeutic e hana i nā hopena immunomodulatory ma o ka hoʻokuʻu ʻana o ka phosphatidylserine.Hōʻailona Transduct Target Ther.2021 Iulai 14;6(1):270.doi: 10.1038/s41392-021-00688-z.

3.IF:17.97 ai Z, Liu H, Liao J, et al.Hoʻonui ka hoʻololi ʻana o N7-Methylguanosine tRNA i ka unuhi oncogenic mRNA a hāpai i ka holomua intrahepatic cholangiocarcinoma.Kelepona Mol.2021 Iulai 29:S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.IF:9.225:Cao X, Shu Y, Chen Y, a me al.Hoʻololi ʻo Mettl14-Mediated m6A i ka hoʻoponopono hou ʻana i ka ate ma o ka mālama ʻana i ka Endoplasmic Reticulum Homeostasis.Cell Mol Gastroenterol Hepatol.2021;12(2):633-651.doi: 10.1016/j.jcmgh.2021.04.001.

 

Nā pahu hoʻokaʻawale RNA no nā kumu hoʻohālike ʻē aʻeloaʻa:

Cell, mea kanu, viral, koko, etc.

---

Mua: Pūnaehana Pūnaewele RT QPCR Kit—SYBR GREEN I

Aʻe: Hoʻopuka ka mea hana i ke kiʻi anti-sun reflection film

---