50 Preps, 200 Preps

Le kit isebenzisa ispin ikholamu kunye nefomulaiphuhliswe yinkampani yethu, enokukhupha ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle okuphezulu.Inika iKholamu yokucoca i-DNA esebenzayo, enokwahlula ngokulula kwaye ibhengeze i-genomic DNA kwi-supernatant kunye ne-tissue lysate, elula. kunye nokonga ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi

Ulwazi lwemveliso

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

 

Amanqaku acatshulweyo

1.UKUBA:18.808:Zheng, Q., Qin, F., Luo, R., et al.I-mRNA-Elayishiweyo iLipid-NjengeNanoparticles yoHlelo oluSiseko seLiver ngokuPhuculwa koYilo oluDityanisiweyo oluPhakathi.Adv.Umsebenzi.Mater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.UKUBA:18.187:Yena X, Hong W, Yang J, et al.I-apoptosis ezenzekelayo yeeseli ekulungiseleleni i-stem cell zonyango zenza i-immunomodulatory iziphumo ngokukhululwa kwe-phosphatidylserine.Ukuhanjiswa kweSignali ekuJoliswe kuyo.2021 Jul 14;6(1):270.doi: 10.1038/s41392-021-00688-z.

3.UKUBA: 17.97 ai Z, Liu H, Liao J, et al.Ukuguqulwa kwe-N7-Methylguanosine tRNA kwandisa uguqulelo lwe-oncogenic mRNA kwaye ikhuthaza ukuqhubeka kwe-intrahepatic cholangiocarcinoma.Iseli yeMol.2021 Jul 29:S1097-2765 (21) 00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.UKUBA:9.225:Cao X, Shu Y, Chen Y, et al.Ukuguqulwa kwe-M6A ye-Mettl14-Mediated Iququzelela ukuHlaziywa kwesibindi ngokuGcina i-Endoplasmic Reticulum Homeostasis.Iseli Mol Gastroenterol Hepatol.2021;12(2):633-651.doi: 10.1016/j.jcmgh.2021.04.001.

 

RNA isoaltion kits for eminye imithombo yesampuluziyafumaneka:

Iseli, isityalo, intsholongwane, igazi, njl.

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Ngaphambili: ISeli eNgqo ngqo yeRT QPCR Kit—SYBR GREEN I

Okulandelayo: Umenzi uvelisa ifilimu echasene nelanga

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