Enzvme detection technology of Enterococcus For water testing
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Character |
This product is white or light yellow particles |
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Clarity |
Colorless or light yellow |
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PH |
7.0 – 7.6 |
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Weight |
2.7 士 0.5g |
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Storage |
at 4°C – 8°C, In cool dry place and protect from ligh |
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Validity |
1 Year, See reagent packaging for production date and expiration date. |
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Science |
Add water sample containing Enterococcus bacteria, culture the target bacteria in Mug medium at 41°C 土 0.5°C, and the specific bological enzymes produced by Enterococcus bacteria(β-D-glucosidase can decompose the fluorescent substrate mug in the mug medium to produce(β-D-glucoside ((β-D-glucoside) and the characteristic fluorescent product 4-metl umbelliferone. Observe the fluorescence in the 366nm UV lamp, count through the quantitative detection disk, and query the MPN table to calculate the results. |
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Package |
100 – test pack |
Usage method Qualitative test
Setp1:Take 100ml water sample with 100ml sterile sampling bottle/ quantitative bottle, add reagent,dissolve, and culture for 24h at 41°C 士 0 . 5°C.
Setp2:Result judgment
Non fluorescence = Negative results Blue fluorescence = Positive results
Quantitative detection
1.Use 100ml sterile sampling bottle to measure 100ml water sample.
2.Add reagent, dissolve it, pour it into 51 Quanti Tray or 97 Quanti-Tray
3.Package the Quanti-Tray with a program controlled quantitative sealing machine, and incubate it at 41°C 士 0 .5 ° C for 24h.
4.Refer to the qualitative test for the result interpretation, and check the MPN table for counting.